Korsnes Biocomputing (KoBio)
KoBio is a startup participating in experimental work on computational methods in biology. ContactLooking for beta testers of our cell tracking software:
We look for partners that can contribute as beta-testers as users of our cell tracking software. We can make a cell visualization product from your live cell video. This includes the ability to browse your video in different ways such as locating special cells, find cells in the video by clicking on a pedigree tree, locate "relative cells" (see screenshot below where four circles mark individual cells with a common second generation ancestor), you get a large set of pedigree trees on a "web solution" which sorts the trees according actual criteria (for example "tree size"). You are supposed to provide us video records of mono-layer cells. You are also supposed patiently to comment on a user manual which we work on and in this way contribute to it. We have to agree on the details for this work. If this message is actual for you, please contact us.Here is an illustration (screenshot) from the use of Kobio_Celltrack (click to make it larger):
News :
- 2016-09-27: KoBio releases its first beta/experimental version of Kobio_Celltrack, which is a portable, free and open source software tool for tracking cells from image recordings of unilayer cells. It based on Ada 2012 using GLOBE_3D as an OpenGL language binding. The system also uses Generic Image Decoder to read image data. This release is considered as a test release. More news and documentation on Kobio_Celltrack to come. Download. *Update: this version is deprecated due to restructuring/rewriting of code.
- 2017-10-02: After another year of work on our cell tracking software, we are now looking for partners that can contribute as beta-testers for it. The software has been, almost totally, rewritten based on acquired experience the latest year.
- 2018-07-06: New publication much based on tracking A549 lung cancer cells during four days: available here .
- 2018-07-16: Have introduced Ada multitasking in tracking program.
Example from multi-tracking of cells using Kobio Celltrack.
The image recording was made by
Cytation 5 Cell Imaging Multi-Mode Reader
from Biotek.
10 hours tracking of many
BC3H1 cells
(left) + one of these cells dividing after almost 9 hours (right):
If you have questions about this software,
contact the main developer Reinert Korsnes:
Video clip showing vacuoles passing from mother cell to her daughters through cell division:
These cells are A549 lung cancer cells exposed to 1000 nM yessotoxin. We prepared this video when tracking the cells for the work https://www.frontiersin.org/articles/10.3389/fonc.2018.00260/full. We used 96-multiwell black microplates # 655090 from Greiner Bio-One IT to plate the A549 cells for recording with Cytation 5.
Other cell tracking software:
Related papers:
KORSNES, Monica Suarez; KORSNES, Reinert. Single-cell tracking of A549 lung cancer cells exposed to a marine toxin reveals correlations in pedigree tree profiles. Frontiers in Oncology, 2018, 8: 260. Full text. This work includes supplementary data showing systematically recorded pedigree trees and videos illustrating tracking of cells exposed to yessotoxin at concentrations 200, 500 and 1000 nM. KORSNES, Monica Suarez; KORSNES, Reinert. Mitotic catastrophe in BC3H1 cells following yessotoxin exposure. Frontiers in Cell and Developmental Biology, 2017, 5. Full text. This work includes supplementary data (video) illustrating tracking of control cells (view video) as well as exposed cells (view video). KORSNES, Monica Suarez; KORSNES, Reinert. Lifetime distributions from tracking individual BC3H1 cells subjected to yessotoxin. Frontiers in bioengineering and biotechnology, 2015, 3. Full text. KORSNES, Reinert, et al. Computer-assisted image processing to detect spores from the fungus Pandora neoaphidis. MethodsX, 2016, 3: 231-241. Full text.
